机读格式显示(MARC)

• 000 03346cam a2200421 i 4500

• 001 0000828830

• 003 OCoLC

• 005 20250905193500.0

• 008 220401s2022 sz b 001 0 eng d

• 020 __ |z 9783030942304 |q (electronic bk.)

• 020 __ |z 3030942309 |q (electronic bk.)

• 020 __ |a 3030942295

• 020 __ |a 9783030942298

• 035 __ |a (OCoLC)1308395994

• 040 __ |a YDX |b eng |e rda |c YDX |d GW5XE |d OCLCO |d EBLCP |d N$T |d OCLCF |d UKAHL |d OCLCQ

• 050 _4 |a QP625.N89

• 082 04 |a 572.8633 |2 23

• 090 __ |a Q524/X1

• 093 __ |a Q524 |2 5

• 100 1_ |a Gautam, Akash, |e author

• 245 10 |a DNA and RNA isolation techniques for non-experts / |c Akash Gautam

• 260 __ |a Cham, Switzerland : |b Springer, |c 2022

• 300 __ |a 1 volume ; |c 24 cm.

• 336 __ |a text |b txt |2 rdacontent

• 337 __ |a unmediated |b n |2 rdamedia

• 338 __ |a volume |b nc |2 rdacarrier

• 490 1_ |a Techniques in life science and biomedicine for the non-expert, |x 2367-1122

• 504 __ |a Includes bibliographical references and index

• 505 0_ |a 1-Basic Idea About Nucleic Acids For Non-Experts -- 2-General Steps During Isolation Of DNA And RNA -- 3-Phenol-Chloroform DNA Isolation Method -- 4-RNA Isolation By The Guanidinium-Acid-Phenol Method -- 5-Spin Column-Based Isolation Of Nucleic Acid -- 6-Isolation Of Plasmid DNA By Alkaline Lysis -- 7-DNA Isolation By Hydrophilic Ionic Liquid Treatment -- 8-Lithium Chloride Based Isolation Of DNA And RNA -- 9-Isolation Of Bacteriophage DNA By Peg Method -- 10-DNA Isolation By Chelex Method -- 11-DNA Isolation By Lysozyme And Proteinase K -- 12-Salting-Out Method For Blood Samples -- 13-CTAB Or SDA Based Isolation Of Plant's DNA -- 14-DNA Extraction By Spooling Method -- 15-Magnetic Bead-Based Nucleic Acid Isolation -- 16-Density Gradient-Based Nucleic Acid Isolation -- 17-DNA Extraction From Agarose Gel Though Paper-Strips -- 18-Transformation Or Genetic Modification Of Cells/Organisms -- 19-Gene Cloning And Vectors -- 20-Polymerase Chain Reaction (PCR) -- 21-Southern And Northern Blotting -- 22-Genome Mapping -- 23-Applications Of DNA Sequencing Technologies For Current Research

• 520 __ |a This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others

• 650 _0 |a Nucleotide sequence.

• 650 _0 |a DNA.

• 710 2_ |a Ohio Library and Information Network. |0 https://id.loc.gov/authorities/names/no95058981

• 776 08 |i Print version: |z 3030942295 |z 9783030942298 |w (OCoLC)1287921130

• 830 _0 |a Techniques in life science and biomedicine for the non-expert. |0 https://id.loc.gov/authorities/names/n2016187336 |x 2367-1122

• 856 40 |3 OhioLINK |z Connect to resource |u https://rave.ohiolink.edu/ebooks/ebc2/9783030942304

• 856 40 |3 SpringerLink |z Connect to resource |u https://link.springer.com/10.1007/978-3-030-94230-4

• 856 40 |3 SpringerLink |z Connect to resource (off-campus) |u https://go.ohiolink.edu/goto?url=https://link.springer.com/10.1007/978-3-030-94230-4