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- 000 04088cam a2200457 i 4500
- 008 211221s2022 nyua b 001 0 eng d
- 020 __ |a 9781071620465 |q hardcover
- 020 __ |a 1071620460 |q hardcover
- 020 __ |a 9781071620496 |q paperback
- 020 __ |a 1071620495 |q paperback
- 020 __ |z 9781071620472 |q electronic book
- 020 __ |z 1071620479 |q electronic book
- 035 __ |a (OCoLC)1295134750 |z (OCoLC)1267585731
- 040 __ |a UKMGB |b eng |e rda |c UKMGB |d OCLCO |d OCLCF |d HNK |d UKMGB |d OCLCO |d COD |d YDX |d BDX |d OCLCL
- 050 _4 |a QP609.D4 |b D63 2022
- 245 00 |a DNAzymes : |b methods and protocols / |c edited by Gerhard Steger, Hannah Rosenbach, Ingrid Span.
- 260 __ |a New York, NY : |b Humana Press, |c [2022]
- 300 __ |a xvi, 312 pages : |b illustrations (chiefly color) ; |c 26 cm.
- 336 __ |a text |b txt |2 rdacontent
- 337 __ |a unmediated |b n |2 rdamedia
- 338 __ |a volume |b nc |2 rdacarrier
- 490 1_ |a Methods in molecular biology, |x 1064-3745 ; |v 2439
- 490 1_ |a Springer protocols
- 504 __ |a Includes bibliographical references and index.
- 505 0_ |a A Protocol for Gold Nanoparticle-Assisted Aptamer Selection for a Small Molecule Porphyrin to Develop DNAzyme -- Selection of Aptamer for N-methyl Mesoporphyrin IX to Develop Porphyrin Metalation DNAzyme -- PCR Methods for the Generation of Catalytic ssDNA -- Design of a DNAzyme: Prediction of mRNA Regions Accessible to a DNAzyme -- Fluorescence-Based Kinetic Measurements for RNA-Cleaving DNAzymes -- Stability and Activity of the 10-23 DNAzyme under Molecular Crowding Conditions -- Quantifying the Number and Affinity of Mn2+-Binding Sites with EPR Spectroscopy -- Obtaining Crystals of Nucleic Acids in Complex with the Protein U1A Using the Soaking Method -- Crystallization and Structural Determination of 8-17 DNAzyme -- Solution NMR Spectroscopy as a Tool to Study DNAzyme Structure and Function -- Molecular Modeling and Simulations of DNA and RNA: DNAzyme as a Model System -- Single-Molecule Kinetic Studies of Nucleic Acids by Fo rster Resonance Energy Transfer -- Chemical Dual End-Labeling of Large Ribozymes -- Spin Labeling of Long RNAs via Click Reaction and Enzymatic Ligation -- Preparation of Site-Specifically Spin-Labeled RNA by In Vitro Transcription using an Expanded Genetic Alphabet -- PELDOR Measurements on Nitroxide-Labelled Oligonucleotides -- Sensing Metal Ions with Phosphorothioate-Modified DNAzymes -- Enhancement of Activity for Peroxidase-Mimicking DNAzyme by Covalent Attachment of Hemin to G-quadruplex-Forming Oligonucleotide Using Click Chemistry -- Generation of RNA with 2' ,3' cyclic Phosphates by Deoxyribozyme Cleavage in Frozen Solutions.
- 520 __ |a This volume explores a collection of different protocols for the analysis and characterization of DNAzymes and their functions. The topics covered in this book range from bioinformatics and molecular dynamics simulations for the study or modification of nucleic acids to the descriptions of spectroscopic, fluorescence-based, or crystallographic methods to understand the structure and function of DNAzymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, DNAzymes: Methods and Protocols is a valuable resource for scientists and researchers interested in learning more about this evolving field. .
- 540 __ |a Current copyright fee: GBP19.00 |c 42\0. |5 Uk
- 650 _0 |a Deoxyribonucleases.
- 650 _6 |a De soxyribonucle ases.
- 650 _7 |a Deoxyribonucleases. |2 fast
- 700 1_ |a Steger, Gerhard, |e editor.
- 700 1_ |a Rosenbach, Hannah, |e editor.
- 700 1_ |a Span, Ingrid, |e editor.
- 830 _0 |a Methods in molecular biology (Clifton, N.J.) ; |v v. 2439.
- 830 _0 |a Springer protocols (Series)