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• 000 03878cam a2200493 i 4500

• 001 0000828783

• 003 OCoLC

• 005 20250903220900.0

• 008 220110t20222022nyua b 001 0 eng d

• 020 __ |a 9781071623244 |q (hardback)

• 020 __ |a 1071623249 |q (hardback)

• 020 __ |z 9781071623251 |q (eBook)

• 035 __ |a (OCoLC)1291390833 |z (OCoLC)1291312722 |z (OCoLC)1291364002 |z (OCoLC)1291368909

• 040 __ |a YDX |b eng |e rda |c YDX |d UKMGB |d OCLCO |d OCLCF |d ALM |d HNK |d OCL |d OCLCO |d OCLCL

• 050 _4 |a QP551 |b .P75 2022

• 082 04 |a 572.6 |2 23

• 090 __ |a Q51/X1

• 093 __ |a Q51 |2 5

• 245 00 |a Proteoform identification : |b methods and protocols / |c edited by Liangliang Sun, Xiaowen Liu.

• 260 __ |a New York, NY : |b Humana Press, |c [2022]

• 260 __ |c 2022

• 300 __ |a x, 240 pages : |b illustrations ; |c 26 cm.

• 336 __ |a text |b txt |2 rdacontent

• 337 __ |a unmediated |b n |2 rdamedia

• 338 __ |a volume |b nc |2 rdacarrier

• 490 1_ |a Methods in molecular biology, |x 1064-3745 ; |v 2500

• 490 1_ |a Springer protocols

• 504 __ |a Includes bibliographical references and index.

• 505 0_ |a Proteoforms and Proteoform Families: Past, Present, and Future -- Membrane Ultrafiltration-Based Sample Preparation Method and Sheath-Flow CZE-MS/MS for Top-Down Proteomics -- Size Exclusion Chromatography Strategies and MASH Explorer for Large Proteoform Characterization -- RPLC-RPLC-MS/MS for Proteoform Identification -- Monolithic Materials-Based RPLC-MS for Proteoform Separation and Identification -- Capillary Isoelectric Focusing: Mass Spectrometry Method for the Separation and Online Characterization of Monoclonal Antibody Charge Variants at Intact and Subunit Level -- Proteoform Analysis and Construction of Proteoform Families in Proteoform Suite -- Top-Down Mass Spectrometry Data Analysis Using TopPIC Suite -- Accurate Proteoform Identification and Quantitation Using pTop 2.0 -- Proteoform Identification and Quantification using Intact Protein Database Search Engine ProteinGoggle -- Mass Deconvolution of Top-Down Mass Spectrometry Datasets by FLASHDeconv -- Deconvolving Native and Intact Protein Mass Spectra with UniDec -- Discovery of Unknown Post-Translational Modifications by Top-Down Mass Spectrometry -- Determining Copper and Zinc Content in Superoxide Dismutase using Electron Capture Dissociation under Native Spray Conditions -- Surface-Induced Dissociation for Protein Complex Characterization.

• 520 __ |a This volume discusses the latest mass spectrometry (MS)-based technologies for proteoform identification, characterization, and quantification. Some of the topics covered in this book include sample preparation, proteoform separation, proteoform gas-phase fragmentation, and bioinformatics tools for MS data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Proteoform Identification: Methods and Protocols is a valuable resource for researchers in both academia and the biopharmaceutical industry who are interested in proteoform analysis using MS. .

• 540 __ |a Current copyright fee: GBP19.00 |c 42\0. |5 Uk

• 650 _0 |a Proteomics.

• 650 _0 |a Mass spectrometry.

• 650 _2 |a Proteomics

• 650 _2 |a Mass Spectrometry

• 650 _6 |a Prote omique.

• 650 _6 |a Spectrome trie de masse.

• 650 _7 |a mass spectrometry. |2 aat

• 650 _7 |a Mass spectrometry |2 fast

• 650 _7 |a Proteomics |2 fast

• 700 1_ |a Sun, Liangliang, |e editor.

• 700 1_ |a Liu, Xiaowen |c (Editor of Proteoform identification), |e editor.

• 830 _0 |a Methods in molecular biology (Clifton, N.J.) ; |v v. 2500.

• 830 _0 |a Springer protocols (Series)