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题名/责任者:

Proteoform identification : methods and protocols / edited by Liangliang Sun, Xiaowen Liu.

出版发行项:

New York, NY : Humana Press, [2022]

出版发行项:

2022

ISBN:

9781071623244

ISBN:

1071623249

载体形态项:

x, 240 pages : illustrations ; 26 cm.

丛编说明:

Methods in molecular biology, 1064-3745 ; 2500

丛编说明:

Springer protocols

丛编统一题名:

Methods in molecular biology (Clifton, N.J.) ; v. 2500.

丛编统一题名:

Springer protocols (Series)

附加个人名称:

Sun, Liangliang, editor.

附加个人名称:

Liu, Xiaowen (Editor of Proteoform identification), editor.

论题主题:

Proteomics.

论题主题:

Mass spectrometry.

论题主题:

Proteomics

论题主题:

Mass Spectrometry

论题主题:

Prote omique.

论题主题:

Spectrome trie de masse.

论题主题:

mass spectrometry.

论题主题:

Mass spectrometry

论题主题:

Proteomics

中图法分类号:

Q51

书目附注:

Includes bibliographical references and index.

内容附注:

Proteoforms and Proteoform Families: Past, Present, and Future -- Membrane Ultrafiltration-Based Sample Preparation Method and Sheath-Flow CZE-MS/MS for Top-Down Proteomics -- Size Exclusion Chromatography Strategies and MASH Explorer for Large Proteoform Characterization -- RPLC-RPLC-MS/MS for Proteoform Identification -- Monolithic Materials-Based RPLC-MS for Proteoform Separation and Identification -- Capillary Isoelectric Focusing: Mass Spectrometry Method for the Separation and Online Characterization of Monoclonal Antibody Charge Variants at Intact and Subunit Level -- Proteoform Analysis and Construction of Proteoform Families in Proteoform Suite -- Top-Down Mass Spectrometry Data Analysis Using TopPIC Suite -- Accurate Proteoform Identification and Quantitation Using pTop 2.0 -- Proteoform Identification and Quantification using Intact Protein Database Search Engine ProteinGoggle -- Mass Deconvolution of Top-Down Mass Spectrometry Datasets by FLASHDeconv -- Deconvolving Native and Intact Protein Mass Spectra with UniDec -- Discovery of Unknown Post-Translational Modifications by Top-Down Mass Spectrometry -- Determining Copper and Zinc Content in Superoxide Dismutase using Electron Capture Dissociation under Native Spray Conditions -- Surface-Induced Dissociation for Protein Complex Characterization.

摘要附注:

This volume discusses the latest mass spectrometry (MS)-based technologies for proteoform identification, characterization, and quantification. Some of the topics covered in this book include sample preparation, proteoform separation, proteoform gas-phase fragmentation, and bioinformatics tools for MS data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Proteoform Identification: Methods and Protocols is a valuable resource for researchers in both academia and the biopharmaceutical industry who are interested in proteoform analysis using MS. .

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