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Online Public Access Catalogue

题名/责任者:

DNA manipulation and analysis / edited by Garry Scarlett.

出版发行项:

New York, NY : Humana Press, [2023]

ISBN:

9781071630037

ISBN:

1071630032

载体形态项:

x, 249 pages : illustrations (some color) ; 26 cm.

其他载体形态:

Online version: DNA manipulation and analysis. New York, NY : Humana Press, 2023 9781071630044

丛编说明:

Methods in molecular biology, 1064-3745 ; 2633

丛编说明:

Springer protocols

丛编统一题名:

Methods in molecular biology (Clifton, N.J.) ; v. 2633.

丛编统一题名:

Springer protocols (Series)

附加个人名称:

Scarlett, Garry, editor.

论题主题:

DNA-Analysis.

论题主题:

DNA replication.

中图法分类号:

Q523

书目附注:

Includes bibliographical references and index.

内容附注:

Classical Recombinant DNA Cloning -- A Sequence and Ligation-Independent Cloning (SLIC) Procedure for the Insertion of Genes into a Plasmid Vector -- Molecular cloning using in vivo DNA assembly -- Assembling multiple fragments: The Gibson Assembly -- TA cloning approaches to cloning DNA with damaged ends DNA -- PCR-based Assembly of Gene Sequences by Thermodynamically Balanced Inside-Out (TBIO) Gene Synthesis -- Random Mutagenesis by PCR -- In vitro Site Directed Mutagenesis -- Xenopus transgenesis using the pGateway system -- CRISPR/Cas9 gene disruption studies in F0 Xenopus tadpoles: understanding development and disease in the frog -- A CRISPR/Cas based method for precise DNA integration in Xenopus laevis oocytes followed by intracytoplasmic sperm injection (ICSI) fertilisation -- A Lambda-Exonuclease SELEX Method for Generating Aptamers to Bacterial Targets. -- Generation of functional-RNA arrays by in vitro transcription and in situ RNA capture for the detection of RNA-RNA interactions -- Chemical synthesis of oligonucelotide sequences: Phosphoramidite chemistry -- Low Throughput Direct Cycle Sequencing of Polymerase Chain Reaction (PCR) Products -- Next generation Sequencing for mixed samples -- Ethics, Legality and Safety for Geneticists.

摘要附注:

This volume details technologies on recombinant DNA and nucleic acid manipulation that underpin much of the biological sciences and instructions on how to conduct them successfully. Chapters guide the reader through the basics of oligonucleotide synthesis and DNA sequencing; recombinant DNA plasmid work; cell-free experiments and the latest developments in CRISPR approaches to genome modification. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, DNA Manipulation and Analysis aims to be the comprehensive guide for life scientists moving into the field of recombinant DNA and nucleic acid manipulation.

豆瓣简介：