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Online Public Access Catalogue

题名/责任者:

DNA and RNA isolation techniques for non-experts / Akash Gautam

出版发行项:

Cham, Switzerland : Springer, 2022

ISBN:

3030942295

ISBN:

9783030942298

载体形态项:

1 volume ; 24 cm.

其他载体形态:

Print version: 3030942295 9783030942298

丛编说明:

Techniques in life science and biomedicine for the non-expert, 2367-1122

丛编统一题名:

Techniques in life science and biomedicine for the non-expert.

个人责任者:

Gautam, Akash, author

附加团体名称:

Ohio Library and Information Network.

论题主题:

Nucleotide sequence.

论题主题:

DNA.

中图法分类号:

Q524

书目附注:

Includes bibliographical references and index

内容附注:

1-Basic Idea About Nucleic Acids For Non-Experts -- 2-General Steps During Isolation Of DNA And RNA -- 3-Phenol-Chloroform DNA Isolation Method -- 4-RNA Isolation By The Guanidinium-Acid-Phenol Method -- 5-Spin Column-Based Isolation Of Nucleic Acid -- 6-Isolation Of Plasmid DNA By Alkaline Lysis -- 7-DNA Isolation By Hydrophilic Ionic Liquid Treatment -- 8-Lithium Chloride Based Isolation Of DNA And RNA -- 9-Isolation Of Bacteriophage DNA By Peg Method -- 10-DNA Isolation By Chelex Method -- 11-DNA Isolation By Lysozyme And Proteinase K -- 12-Salting-Out Method For Blood Samples -- 13-CTAB Or SDA Based Isolation Of Plant's DNA -- 14-DNA Extraction By Spooling Method -- 15-Magnetic Bead-Based Nucleic Acid Isolation -- 16-Density Gradient-Based Nucleic Acid Isolation -- 17-DNA Extraction From Agarose Gel Though Paper-Strips -- 18-Transformation Or Genetic Modification Of Cells/Organisms -- 19-Gene Cloning And Vectors -- 20-Polymerase Chain Reaction (PCR) -- 21-Southern And Northern Blotting -- 22-Genome Mapping -- 23-Applications Of DNA Sequencing Technologies For Current Research

摘要附注:

This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others

豆瓣简介：